OMA1 Monoclonal antibody

OMA1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4D12

Cat no : 67449-1-Ig

Synonyms

2010001O09Rik, DAB1, FLJ33782, MPRP 1, MPRP1, OMA1, YKR087C, ZMPOMA1

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 67449-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67449-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67449-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67449-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67449-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, Jurkat cells, LNCaP cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67449-1-Ig targets OMA1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OMA1 fusion protein Ag11201
• Full Name: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Calculated Molecular Weight: 524 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012915
• Gene Symbol: OMA1
• Gene ID (NCBI): 115209
• RRID: AB_2882683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OMA1(Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1(Metalloprotease-related protein 1) and belongs to the peptidase M48 family.It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants,and the 40 kDa form of OMA1 may be the inactive form, with the 60 kDa form being the active enzyme(PMID:20334834)