OGDHL Recombinant antibody
OGDHL Recombinant Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
230228B2
Cat no : 82981-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82981-1-RR targets OGDHL in IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | OGDHL fusion protein Ag10794 |
Full Name | oxoglutarate dehydrogenase-like |
Calculated Molecular Weight | 1010 aa, 115 kDa |
GenBank Accession Number | BC026320 |
Gene Symbol | OGDHL |
Gene ID (NCBI) | 55753 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.
Protocols
Product Specific Protocols | |
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IHC protocol for OGDHL antibody 82981-1-RR | Download protocol |
IF protocol for OGDHL antibody 82981-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |