CoraLite® Plus 488-conjugated OGDH Monoclonal antibody

OGDH Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7A2

Cat no : CL488-66285

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-66285

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:200, CL594-phalloidin (red).

• 

  IF Staining of L02 using CL488-66285

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite® Plus 488-conjugated OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:100, CL594-Phalloidin (red).

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells, L02 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66285 targets OGDH in IF/ICC applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: OGDH fusion protein Ag7484
• Full Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC004964
• Gene Symbol: OGDH
• Gene ID (NCBI): 4967
• RRID: AB_2883303
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.