Tested Applications
| Positive WB detected in | human heart tissue, ROS1728 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66285-1-Ig targets OGDH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7484 Product name: Recombinant human OGDH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 674-1023 aa of BC004964 Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS Predict reactive species |
| Full Name | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | BC004964 |
| Gene Symbol | OGDH |
| Gene ID (NCBI) | 4967 |
| RRID | AB_2881668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02218 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OGDH antibody 66285-1-Ig | Download protocol |
| IHC protocol for OGDH antibody 66285-1-Ig | Download protocol |
| WB protocol for OGDH antibody 66285-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OGDH Monoclonal antibody (1H7A2), catalog number 66285-1-Ig, has 6 citations published in Nature Communications, Nature Cardiovascular Research, Cell Reports, Chinese Medicine, Human Molecular Genetics, and PLoS One. The research fields span mitochondrial metabolism and AMPylation, cardiomyocyte proliferation and heart regeneration, beta cell dysfunction in type 1 diabetes, energy metabolism in cerebral ischemia-reperfusion, 2-oxo acid dehydrogenase complex biology, and pancreatic cancer treatment targeting lactate metabolism.
| Species | Application | Title |
|---|---|---|
Nat Commun A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism | ||
Nat Cardiovasc Res α-Ketoglutarate promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction | ||
Cell Rep Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis. | ||
Chin Med Buyang Huanwu Decoction improves energy metabolism disorders after cerebral ischemia-reperfusion by regulating the SIRT1/AMPK signaling pathway to promote glycolysis and the tricarboxylic acid cycle | ||
Hum Mol Genet DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. | ||
PLoS One Targeting pancreatic cancer with combinatorial treatment of CPI-613 and inhibitors of lactate metabolism. |
