OGDH Monoclonal antibody, PBS Only

OGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7A2

Cat no : 66285-1-PBS

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH

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Validation Data Gallery

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  WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66285-1-Ig (same clone as 66285-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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Product Information

66285-1-PBS targets OGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: OGDH fusion protein Ag7484
• Full Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC004964
• Gene Symbol: OGDH
• Gene ID (NCBI): 4967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.