Nurr1/NR4A2 Monoclonal antibody, PBS Only

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  WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66878-1-Ig (same clone as 66878-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66878-1-Ig (same clone as 66878-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66878-1-Ig (same clone as 66878-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Nurr1/NR4A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66878-1-PBS

Clone No.1H5B4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

NURR1, NR4A2, NOT, Immediate-early response protein NOT, HZF 3

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Product Information

66878-1-PBS targets Nurr1/NR4A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Full Name: nuclear receptor subfamily 4, group A, member 2
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC009288
• Gene Symbol: Nurr1
• Gene ID (NCBI): 4929
• RRID: AB_2882211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43354
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].