Nurr1/NR4A2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Nurr1/NR4A2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1H5B4

Cat no : 66878-1-Ig

Synonyms

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

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Validation Data Gallery

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  WB analysis using 66878-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66878-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of HT-29 using 66878-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66878-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66878-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66878-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66878-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, Ramos cells, Daudi cells, HT-29 cells, Raji cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66878-1-Ig targets Nurr1/NR4A2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Full Name: nuclear receptor subfamily 4, group A, member 2
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC009288
• Gene Symbol: Nurr1
• Gene ID (NCBI): 4929
• RRID: AB_2882211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

Publications

Species	Application	Title
human	WB	Front Mol Biosci; Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.; Authors - Wenzhou Liu ; KD Validated
rat	WB,IHC	Mol Cell Neurosci; BDNF-modified human umbilical cord mesenchymal stem cells-derived dopaminergic-like neurons improve rotation behavior of Parkinson's disease rats through neuroprotection and anti-neuroinflammation; Authors - Zhi Jiang
mouse	WB	World J Gastroenterol; 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota; Authors - Qiong-Zi He
human,rat	WB	J Pers Med; Identification and Validation of Hub Genes in the Stenosis of Arteriovenous Fistula; Authors - Yu Li
rat	IF	J Reprod Dev; Parallel expression patterns of NR4A nuclear receptor family genes in the pituitary gland of proestrus rats; Authors - Ryota Terashima