CoraLite® Plus 488-conjugated Nucleolin/C23 Recombinant antibody

Nucleolin/C23 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240029F8

Cat no : CL488-83380

Synonyms

C23, nucleolin, Protein C23, NCL, FLJ45706

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Validation Data Gallery

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  IF Staining of HeLa using CL488-83380

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Nucleolin/C23 antibody (CL488-83380, Clone: 240029F8 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83380 is Nucleolin/C23 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Nucleolin/C23 fusion protein Ag0859
• Full Name: nucleolin
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC006494
• Gene Symbol: NCL
• Gene ID (NCBI): 4691
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)