NeutraKine®IL-9 Monoclonal antibody

NeutraKine®IL-9 Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

1B3A10

Cat no : 69031-1-Ig

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40

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Validation Data Gallery

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  Neutralization experiment of NeutraKine®IL-9 using 69031-1-Ig

  Recombinant human IL-9 (Cat.NO. HZ-1240) stimulates proliferation of the MO7e human megakaryoblastic leukemia cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-9 (0.8 ng/mL HZ-1240) is neutralized by mouse anti-human IL-9 monoclonal antibody 69031-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.2-1.0 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis).

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Neutralization

Product Information

69031-1-Ig targets NeutraKine®IL-9 in ELISA, Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: human HumankineIL-9 protein HZ-1240
• Full Name: interleukin 9
• Gene Symbol: IL9
• Gene ID (NCBI): 3578
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.

This antibody can be used to neutralize the bioactivity of IL-9.