NeutraKine® IL-3 Monoclonal antibody, PBS Only

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  Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig (same clone as 69004-1-PBS)

  Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (10 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 40-70 ng/mL. This data was developed using the same antibody clone with 69004-1-PBS in a different storage buffer formulation.

• 

  Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig (same clone as 69004-1-PBS)

  Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (0.5 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.8-2.4 ng/mL. This data was developed using the same antibody clone with 69004-1-PBS in a different storage buffer formulation.

NeutraKine® IL-3 Monoclonal Antibody for Neutralization, ELISA

Cat No. 69004-1-PBS

Clone No.3C11G7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA

Synonyms

IL3, IL-3, 3C11G7, IL 3, Interleukin 3

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Product Information

69004-1-PBS targets NeutraKine® IL-3 in Neutralization, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-3 protein HZ-1074
• Full Name: interleukin 3 (colony-stimulating factor, multiple)
• Gene Symbol: IL-3
• Gene ID (NCBI): 3562
• Conjugate: Unconjugated
• Purification Method: Protein G purification
• UNIPROT ID: P08700
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196).

This antibody can be used to neutralize the bioactivity of IL-3.