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NeutraKine® IL-3 Monoclonal antibody

NeutraKine® IL-3 Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

3C11G7

Cat no : 69004-1-Ig

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, NeutraKine® IL-3, P cell stimulating factor

Validation Data Gallery

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  • Neutralization experiment of NeutraKine® IL-3 using NeutraKine® IL-3 Monoclonal antibody (69004-1-Ig)

    Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig

    Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (10 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 40-70 ng/mL.

  • Neutralization experiment of NeutraKine® IL-3 using NeutraKine® IL-3 Monoclonal antibody (69004-1-Ig)

    Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig

    Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (0.5 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.8-2.4 ng/mL.

Neutralization

Product Information

69004-1-Ig targets NeutraKine® IL-3 in ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-3 protein HZ-1074
Full Name interleukin 3 (colony-stimulating factor, multiple)
Gene Symbol IL-3
Gene ID (NCBI) 3562
RRIDAB_2882922
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196).

This antibody can be used to neutralize the bioactivity of IL-3.