NeutraKine®FGF basic Monoclonal antibody

NeutraKine®FGF basic Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

ELISA, Neutralization, Cell treatment

Conjugate

Unconjugated

Publications

1

CloneNo.

3C9C3

Cat no : 69024-1-Ig

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF2, FGFB, FGFbasic, HBGF 2

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Validation Data Gallery

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  Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig

  Recombinant human FGF Basic (Cat.NO. HZ-1285) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) at low FBS condition in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FGF Basic (0.1 ng/mL HZ-1285) is neutralized by mouse anti-human FGF Basic monoclonal antibody 69024-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 1-10 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis).

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Neutralization

Product Information

69024-1-Ig targets NeutraKine®FGF basic in ELISA, Neutralization, Cell treatment applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human HumankineFGF basic protein HZ-1285
• Full Name: fibroblast growth factor 2 (basic)
• Gene Symbol: FGF2
• Gene ID (NCBI): 2247
• RRID: AB_2918844
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

This antibody is used to neutralize the bioactivity of FGFbasic-TS.

Publications

Species	Application	Title
human	Cell treatment	Int Immunopharmacol; Cancer-associated fibroblasts promote gastric cancer cell proliferation by paracrine FGF2-driven ribosome biogenesis; Authors - Dandan Li