NeutraControl IL-27 Monoclonal antibody

NeutraControl IL-27 Monoclonal Antibody for ELISA, Non-Neutralization

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA, Non-Neutralization

Conjugate

Unconjugated

CloneNo.

2F8E2

Cat no : 69534-1-Ig

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

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Validation Data Gallery

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  Non-Neutralization experiment of OCI-AML5 cells using 69534-1-Ig

  Recombinant human IL-27(Cat.NO. HZ-1275) stimulates proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-27 (250 ng/mL) is neutralized by mouse anti-human IL-27 monoclonal antibody 69034-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.4-1 μg/mL.The control mouse anti-human IL-27 monoclonal antibody 69534-1-Ig could recognize human IL-27, but could not neutralize human IL-27 at serial does at the range of 0.0033-9μg/mL(green curve, refer to top X-right Y).

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  ELISA experiment of Recombinant protein using 69534-1-Ig

  Indirect ELISA was carried out by coating recombinant Human IL-27 (Cat.NO. HZ-1275) at 70 ng/well followed by blocking and adding serial diluted IL-27 antibody 69034-1-Ig and 69534-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Product Information

69534-1-Ig targets IL-27 in ELISA, Non-Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-27 protein HZ-1275
• Full Name: interleukin 27
• Gene Symbol: IL-27
• Gene ID (NCBI): 246778
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.

This antibody is a neutralizing control antibody for IL-27, the immunogen is the same as Neutrakine 69034-1-Ig but could not neutralize human IL-27.