HumanKine® recombinant human IL-27 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

15-200 ng/mL in OCI-AML5 cells

Species Reactivity

human

Purity

>95%

Publications

2

Cat no : HZ-1275

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

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Validation Data Gallery

• 

  Purity of recombinant human IL-27 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human IL-27 (HZ-1275) stimulates dose-dependent proliferation of the OCI-AML5 (human acute myeloid leukemia) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human IL-27 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 15-200 ng/mL.

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Technical Specifications

• GeneID: 246778
• Species: Human
• Expression: HEK293
• Activity(Primary): 15-200 ng/mL in OCI-AML5 cells
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: Q8NEV9
• Molecular Mass: 25 and 30 kDa reduced and non-reduced, heterodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases (PMID: 22608254;16081786;16670296;16906167)

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

Publications

Species	Title
human	J Exp Med; IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation.; Authors - LueDai
human	Stem Cell Res Ther; hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.; Authors - Aiping Zhang