Neudesin/NENF Monoclonal antibody, PBS Only (Capture)

Neudesin/NENF Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G9E12

Cat no : 60131-1-PBS

Synonyms

Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor, Cell immortalization-related protein 2

---

Validation Data Gallery

• 

  WB analysis using 60131-1-Ig (same clone as 60131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 60131-1-Ig (same clone as 60131-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1900 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60131-1-Ig(NENF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50685-1

  Cytometric bead array standard curve of MP50685-1, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-2-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

• 

  Cytometric bead array standard curve of MP50685-2

  Cytometric bead array standard curve of MP50685-2, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-3-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

---

Product Information

60131-1-PBS targets Neudesin/NENF as part of a matched antibody pair:

MP50685-1: 60131-1-PBS capture and 60131-2-PBS detection (validated in Cytometric bead array)

MP50685-2: 60131-1-PBS capture and 60131-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Neudesin/NENF fusion protein Ag8387
• Full Name: neuron derived neurotrophic factor
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 19 kDa, 16 kDa
• GenBank Accession Number: BC008823
• Gene Symbol: NENF
• Gene ID (NCBI): 29937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).