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CoraLite®555-conjugated NeuN Polyclonal antibody

NeuN Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-26975

Synonyms

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®555-conjugated NeuN Polyclonal antibody (CL555-26975)

    IF Staining of mouse brain using CL555-26975

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®555 NeuN antibody (CL555-26975) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U-87 MG cells using CoraLite®555-conjugated NeuN Polyclonal antibody (CL555-26975)

    FC experiment of U-87 MG using CL555-26975

    1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human NeuN (CL555-26975) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inU-87 MG cells
Positive FC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-26975 targets NeuN in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NeuN fusion protein Ag25689
Full Name hexaribonucleotide binding protein 3
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2919647
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).


Protocols

Product Specific Protocols
IF protocol for CL555 NeuN antibody CL555-26975Download protocol
Standard Protocols
Click here to view our Standard Protocols