NeuN Monoclonal antibody

NeuN Monoclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat and More (2)







Cat no : 66836-1-Ig


cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

Tested Applications

Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue, rat cerebellum tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66836-1-Ig targets NeuN in IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NeuN fusion protein Ag28016
Full Name hexaribonucleotide binding protein 3
GenBank Accession NumberNM_001082575
Gene Symbol hCG_1776007
Gene ID (NCBI) 146713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.


Product Specific Protocols
IHC protocol for NeuN antibody 66836-1-IgDownload protocol
IF protocol for NeuN antibody 66836-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.

Authors - Weiping Xiao

Cell Death Dis

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy

Authors - Yang Deng

Cell Death Dis

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Authors - Yaxuan Zhang

Environ Int

Polystyrene micro- and nano-particle coexposure injures fetal thalamus by inducing ROS-mediated cell apoptosis.

Authors - Diqi Yang


Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain

Authors - Shahzad S Khan

Antioxidants (Basel)

Epimedium Aqueous Extract Ameliorates Cerebral Ischemia/Reperfusion Injury through Inhibiting ROS/NLRP3-Mediated Pyroptosis

Authors - Xiaoyu Wu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kenzo (Verified Customer) (01-05-2024)

This monoclonal NeuN worked well for mouse tissues.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Retina

Silvia (Verified Customer) (08-11-2022)

it works well for Immunofluorescence on mature neurons

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: hiPSCs-derived neurons

Delphine (Verified Customer) (07-25-2022)

Immunohistochemistry with the NeuN antibody on frozen spinal cord worked but the labeling must be optimized because there is a lot of background noise.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: tissue

q (Verified Customer) (01-05-2022)

It is OK to use it in WB, but several non-specific bands above the expected MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
NeuN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66836-1-Ig)