Validation Data Gallery
|Positive IHC detected in||mouse brain tissue, rat brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse cerebellum tissue, rat cerebellum tissue|
|Positive FC detected in||SH-SY5Y cells|
|Immunohistochemistry (IHC)||IHC : 1:10000-1:40000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66836-1-Ig targets NeuN in IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||NeuN fusion protein Ag28016|
|Full Name||hexaribonucleotide binding protein 3|
|GenBank accession number||NM_001082575|
|Gene ID (NCBI)||146713|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
Cell Death Dis
SARM1 deletion in parvalbumin neurons is associated with autism-like behaviors in mice.
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Establishment and Evaluation of a Novel High-efficiency Model of Graded Traumatic Brain Injury in Mice.
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Repair of Spinal Cord Injury by Inhibition of PLK4 Expression Through Local Delivery of siRNA-Loaded Nanoparticles.
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Lysosomal dysfunction is associated with NLRP3 inflammasome activation in chronic unpredictable mild stress-induced depressive mice.
Protective Effects of Bile Acids Against Hepatic Lipid Accumulation in Hybrid Grouper Fed a High-Lipid Diet.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (08-11-2022)
it works well for Immunofluorescence on mature neurons
Delphine (Verified Customer) (07-25-2022)
Immunohistochemistry with the NeuN antibody on frozen spinal cord worked but the labeling must be optimized because there is a lot of background noise.