Nestin Polyclonal antibody, PBS Only

Nestin Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 29285-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29285-1-AP (same clone as 29285-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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  WB analysis using 29285-1-AP (same clone as 29285-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 29285-1-AP (same clone as 29285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 29285-1-AP (same clone as 29285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), NeuN antibody (66836-1-Ig, Clone: 3A4C1, red). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 29285-1-AP (same clone as 29285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 29285-1-AP (same clone as 29285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 29285-1-AP (same clone as 29285-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Nestin antibody (29285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 29285-1-AP (same clone as 29285-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Nestin (29285-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

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Product Information

29285-1-PBS targets Nestin in WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nestin fusion protein Ag29865
• Full Name: nestin
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 240-280 kDa
• GenBank Accession Number: NM_006617
• Gene Symbol: Nestin
• Gene ID (NCBI): 10763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).