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Nestin Polyclonal antibody

Nestin Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 29285-1-AP

Synonyms

NES

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (29285-1-AP)

    WB analysis using 29285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (29285-1-AP)

    WB analysis using 29285-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of human kidney using 29285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of rat brain using 29285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), NeuN antibody (66836-1-Ig, Clone: 3A4C1, red).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of rat brain using 29285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of mouse kidney using 29285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of U-251 using 29285-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Nestin antibody (29285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Nestin Polyclonal antibody (29285-1-AP)

    FC experiment of SH-SY5Y using 29285-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Nestin (29285-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inSGC-7901 cells, U-251 cells, U-87 MG cells
Positive IF-P detected inrat brain tissue, mouse kidney tissue, human kidney tissue
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

29285-1-AP targets Nestin in WB, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nestin fusion protein Ag29865
Full Name nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight240-280 kDa
GenBank Accession NumberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_2918268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Protocols

Product Specific Protocols
WB protocol for Nestin antibody 29285-1-APDownload protocol
IF protocol for Nestin antibody 29285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Bioact Mater

Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair

Authors - Zhaoping Wu
View Article
mouse,ratIF

Brain Behav Immun

Microbial reconstitution reverses prenatal stress-induced cognitive impairment and synaptic deficits in rat offspring

Authors - Jie Chen
View Article