Nephrin Recombinant antibody, PBS Only

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  WB analysis of mouse kidney using 85845-1-RR (same clone as 85845-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85845-1-RR (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85845-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85845-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85845-1-RR against Human Nephrin were performed. The affinity constant is below 1 pM.

Nephrin Recombinant Antibody for WB, Indirect ELISA

Cat No. 85845-1-PBS

Clone No.250064G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

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Product Information

85845-1-PBS targets Nephrin in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Nephrin fusion protein Ag18994
• Full Name: nephrosis 1, congenital, Finnish type (nephrin)
• Calculated Molecular Weight: 1241 aa, 135 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC156935
• Gene Symbol: Nephrin
• Gene ID (NCBI): 4868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60500
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).