Anti-Human Nectin-2/CD112 Rabbit Recombinant Antibody

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  FC experiment of K562 using 98283-1-RR

  1x10^6 K562 (left) or Daudi cells (right) were surface stained with 0.25 ug Anti-Human Nectin-2/CD112 Rabbit RecAb (98283-1-RR, Clone: 241725D3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Nectin-2/CD112 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98283-1-RR

Clone No.241725D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Nectin-2, Nectin 2, HVEB, Herpesvirus entry mediator B, Herpes virus entry mediator B

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Tested Applications

• Positive FC detected in: K562

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98283-1-RR targets Nectin-2/CD112 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: NM_001042724.2
• Gene Symbol: Nectin-2/CD112
• Gene ID (NCBI): 5819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q92692-2
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.