NCAM1/CD56 Recombinant antibody

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  IHC staining of mouse brain using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuro-2a using 83883-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Neuro-2a using 83883-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83883-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83883-5-RR against Mouse Ncam1 were performed. The affinity constant is 0.184 nM.

NCAM1/CD56 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 83883-5-RR

Clone No.240936B10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Ncam1, CD56, 240936B10, NCAM 1, Ncam

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Tested Applications

• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83883-5-RR targets NCAM1/CD56 in IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 120 kDa, 140 kDa, 180 kDa
• Gene Symbol: Ncam1
• Gene ID (NCBI): 17967
• RRID: AB_3671465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13595-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).