• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse Ncam1 Recombinant antibody, PBS Only

Ncam1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240936B10

Cat no : 83883-5-PBS

Synonyms

CD56, NCAM 1, Ncam, E NCAM, 240936B10

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using Ncam1 Recombinant antibody (83883-5-RR)

    IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using Ncam1 Recombinant antibody (83883-5-RR)

    IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83883-5-RR

    Affinity and Kinetic Characterization of 83883-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83883-5-RR against Mouse Ncam1 were performed. The affinity constant is 0.184 nM.

Product Information

The immunogen of 83883-5-PBS is Ncam1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name neural cell adhesion molecule 1
Calculated Molecular Weight119 kDa
Observed Molecular Weight120 kDa, 140 kDa, 180 kDa
Gene Symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).