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ChromoTek Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH CoraLite® Plus 488 [CTK0119, CTK0120]

Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.

Applications

WB, IF

Conjugate

CoraLite® Plus 488

Host

Alpaca

Reactivity

Rabbit IgG

Cat no : srb2GCL488-1

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation Data Gallery

  • HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antiboy (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500.

    HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antiboy (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500.

  • Immunofluorescence analysis of A431 cells stained with rabbit anti-NCL antibody (10556-1-AP, green) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500. Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells stained with rabbit anti-NCL antibody (10556-1-AP, green) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500. Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence analysis of A431 cells co-stained with anti-EGFR antibody (cetuximab biosimilar, huIgG1, magenta) and anti-NCL antibody (10556-1-AP, green). Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 (shuGCL647-2), 1:500 and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500 were used for detection. Nuclei were stained with DAPI. Merged image on the right shows an overlay of both channels and DAPI (blue). ​Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells co-stained with anti-EGFR antibody (cetuximab biosimilar, huIgG1, magenta) and anti-NCL antibody (10556-1-AP, green). Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 (shuGCL647-2), 1:500 and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500 were used for detection. Nuclei were stained with DAPI. Merged image on the right shows an overlay of both channels and DAPI (blue). ​Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Product Information

Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.

ApplicationsWB, IF
DescriptionNano-Secondary® alpaca anti-rabbit IgG, recombinant VHH reagent uses a novel class of rabbit IgG-specific antibodies. This secondary antibody product consists of nanobodies that bind to rabbit IgG with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, see details below.
HostAlpaca
Species ReactivityReactivity: Rabbit IgG
No cross-reactivity: mouse, rat and human IgGs.
Physical StateLiquid
ConjugateCoraLite® Plus 488
Suggested DilutionImmunofluorescence 1:500
Western blot: 1:500
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
Affinity (KD)CTK0119: KD = 0.2 nM, CTK120: KD = <10 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage Condition Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
RRIDAB_3094639
NoteThis product is for research use only, not for diagnostic or therapeutic use