Validation Data Gallery
![Dot blot assay demonstrates that Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] has no cross-reactivity to mouse, rat, or human immunoglobulins.](https://www.ptglab.com/products/pictures/Result-srb2GCys2-2.jpg "Dot blot assay demonstrates that Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] has no cross-reactivity to mouse, rat, or human immunoglobulins.")
![The affinity of Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] towards rabbit IgG was determined using biolayer interferometry (BLI). Biotinylated, polyclonal rabbit IgG was immobilized on Streptavidin biosensors and assayed with 0.3 to 10 nM VHH. Fit indicates a 1:1 binding model fitted to the data.](https://www.ptglab.com/products/pictures/Result-srb2GCys2-2_20240226162732555.jpg "The affinity of Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] towards rabbit IgG was determined using biolayer interferometry (BLI). Biotinylated, polyclonal rabbit IgG was immobilized on Streptavidin biosensors and assayed with 0.3 to 10 nM VHH. Fit indicates a 1:1 binding model fitted to the data.")
Product Information
Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides
| Applications | IF, WB, FC |
| Host | Alpaca |
| Reactivity | Rabbit |
| Specificity/Target | Fc-fragment of Rabbit IgG |
| Conjugate | N- and C-terminal cysteine conjugation with thiol-reactive reagents |
| Molecular weight | 14.9 Da |
| Extinction coefficient (280 nm) | 32.680 L * Mol-1 * cm-1 |
| Type | Nano-Secondary® Reagent, secondary Nanobody (VHH) |
| Format | Alpaca single domain antibody, monovalent |
| Cross-reactivity | No cross-reactivity to mouse, rat or human IgGs |
| Immunogen | Purified rabbit IgG |
| Clonality | Monoclonal Nanobody |
| Clone | CTK0119 |
| Affinity | 0.2 nM |
| RRID | AB_3094643 |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C. |
| Size | 500 µg |
