ChromoTek Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119]

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

Applications

IF, WB, FC

Conjugate

2x Cys

Host

Alpaca

Reactivity

Rabbit

Cat no : srb2GCys2-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

---

Validation Data Gallery

• 

  Dot blot assay demonstrates that Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] has no cross-reactivity to mouse, rat, or human immunoglobulins.

• 

  The affinity of Nano-Secondary® alpaca anti-rabbit IgG Fc, recombinant VHH, for 2x Cys conjugation [CTK0119] towards rabbit IgG was determined using biolayer interferometry (BLI). Biotinylated, polyclonal rabbit IgG was immobilized on Streptavidin biosensors and assayed with 0.3 to 10 nM VHH. Fit indicates a 1:1 binding model fitted to the data. 

---

Product Information

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

• Applications: IF, WB, FC
• Clonality: Monoclonal Nanobody
• Clone: CTK0119
• Host: Alpaca
• Affinity: 0.2 nM
• Specificity/Target: Fc-fragment of Rabbit IgG
• Reactivity: Rabbit
• Conjugate: N- and C-terminal cysteine conjugation with thiol-reactive reagents
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C.
• RRID: AB_3094643