NVL Monoclonal antibody

NVL Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68399-1-Ig
Clone No.1D8G4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NVL fusion protein Ag9321 Predict reactive species
Full Name nuclear VCP-like
Calculated Molecular Weight856aa,95 kDa; 659aa,73 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC012105
Gene Symbol NVL
Gene ID (NCBI) 4931
RRIDAB_3085116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NVL antibody 68399-1-IgDownload protocol
FC protocol for NVL antibody 68399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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