NUP85 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00330-1

  Sandwich ELISA standard curve of MP00330-1, Human NUP85 Recombinant Matched Antibody Pair - PBS only. 83288-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7086. 83288-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00330-1

  The mean NUP85 concentration was determined to be 1,874.5 pg/mL in mouse liver tissue extract based on a 3.0 mg/mL extract load.

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  FC experiment of MCF-7 using 83288-2-RR (same clone as 83288-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-2-RR, Clone:240116E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00330-1

  Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00330-2

  Cytometric bead array standard curve of MP00330-2, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-3-PBS. Standard: Ag7086. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83288-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83288-2-RR against Human NUP85 were performed. The affinity constant is 27.3 nM.

NUP85 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83288-2-PBS

Clone No.240116E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

240116E10, 85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75

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Product Information

83288-2-PBS targets NUP85 as part of a matched antibody pair:

MP00330-1: 83288-2-PBS capture and 83288-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00330-2: 83288-2-PBS capture and 83288-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NUP85 fusion protein Ag7086
• Full Name: nucleoporin 85kDa
• Calculated Molecular Weight: 75 kDa
• GenBank Accession Number: BC000697
• Gene Symbol: NUP85
• Gene ID (NCBI): 79902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BW27
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.