NUP85 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00330-1

  Sandwich ELISA standard curve of MP00330-1, Human NUP85 Recombinant Matched Antibody Pair - PBS only. 83288-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7086. 83288-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00330-1

  The mean NUP85 concentration was determined to be 1,874.5 pg/mL in mouse liver tissue extract based on a 3.0 mg/mL extract load.

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  FC experiment of MCF-7 using 83288-2-RR (same clone as 83288-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-2-RR, Clone:240116E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00330-1

  Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83288-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83288-2-RR against Human NUP85 were performed. The affinity constant is 27.3 nM.

NUP85 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83288-2-PBS

Clone No.240116E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

240116E10, 85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75

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Product Information

83288-2-PBS targets NUP85 as part of a matched antibody pair:

MP00330-1: 83288-2-PBS capture and 83288-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NUP85 fusion protein Ag7086
• Full Name: nucleoporin 85kDa
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC000697
• Gene Symbol: NUP85
• Gene ID (NCBI): 79902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BW27
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.