CoraLite®594-conjugated NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C4C8

Cat no : CL594-66335

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66335

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66335 (NUDT21 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66335 targets NUDT21 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NUDT21 fusion protein Ag12260
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001403
• Gene Symbol: NUDT21
• Gene ID (NCBI): 11051
• RRID: AB_2883561
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.