NUCB2 Recombinant antibody, PBS Only

NUCB2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241037C1

Cat no : 83917-5-PBS

Synonyms

NUCB2/nesfatin-1, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

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Validation Data Gallery

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  IF Staining of HepG2 using 83917-5-RR (same clone as 83917-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2 antibody (83917-5-RR, Clone: 241037C1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83917-5-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83917-5-RR (same clone as 83917-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83917-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83917-5-RR (same clone as 83917-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83917-5-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83917-5-PBS is NUCB2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NUCB2 fusion protein Ag25152
• Full Name: nucleobindin 2
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: NM_005013
• Gene Symbol: NUCB2
• Gene ID (NCBI): 4925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).