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NUCB2/nesfatin-1 Recombinant antibody

NUCB2/nesfatin-1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241037C1

Cat no : 83917-5-RR

Synonyms

NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUCB2 Recombinant antibody (83917-5-RR)

    IF Staining of HepG2 using 83917-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2 antibody (83917-5-RR, Clone: 241037C1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using NUCB2 Recombinant antibody (83917-5-RR)

    FC experiment of A431 using 83917-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using NUCB2 Recombinant antibody (83917-5-RR)

    FC experiment of HepG2 using 83917-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83917-5-RR is NUCB2/nesfatin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUCB2/nesfatin-1 fusion protein Ag25152
Full Name nucleobindin 2
Calculated Molecular Weight 50 kDa
GenBank Accession NumberNM_005013
Gene Symbol NUCB2
Gene ID (NCBI) 4925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Protocols

Product Specific Protocols
IF protocol for NUCB2/nesfatin-1 antibody 83917-5-RRDownload protocol
FC protocol for NUCB2/nesfatin-1 antibody 83917-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols