NTRK1 Recombinant antibody

NTRK1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240440D12

Cat no : 83477-7-RR

Synonyms

TRK1, Trk A, TRK, p140 TrkA, MTC

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Validation Data Gallery

• 

  WB analysis of K-562 using 83477-7-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83477-7-RR (NTRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 83477-7-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83477-7-RR (NTRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83477-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83477-7-RR against Human NTRK1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: K-562 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83477-7-RR is NTRK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NTRK1 fusion protein Ag32759
• Full Name: neurotrophic tyrosine kinase, receptor, type 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001007792
• Gene Symbol: NTRK1
• Gene ID (NCBI): 4914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTRK1(Neurotrophic tyrosine kinase receptor 1) is located on chromosome 1q23.1 and is responsible for a rare genetic disorder, CIPA (congenital insensitivity to pain with anhidrosis) (PMID: 37907480). As a neurotrophin receptor, NTRK1 is abundantly expressed in human neuronal tissues and plays an integral role in the development and function of the human nervous system (PMID: 27843590). NTRK1 also regulates the survival and proliferation of cholinergic neurons (PMID: 20054812).