NTF2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NTF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7F9

Cat no : 66063-1-PBS

Synonyms

NTF 2, NTF2, nuclear transport factor 2, NUTF 2, NUTF2, Placental protein 15, PP15

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Validation Data Gallery

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  WB analysis of HeLa using 66063-1-Ig (same clone as 66063-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66063-1-Ig (same clone as 66063-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66063-1-Ig (same clone as 66063-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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Product Information

66063-1-PBS targets NTF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NTF2 fusion protein Ag7879
• Full Name: nuclear transport factor 2
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC002348
• Gene Symbol: NTF2
• Gene ID (NCBI): 10204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.