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  • Featured Product
  • KD/KO Validated

NTF2 Monoclonal antibody

NTF2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4H7F9

Cat no : 66063-1-Ig

Synonyms

NTF 2, NTF2, nuclear transport factor 2, NUTF 2, NUTF2, Placental protein 15, PP15

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NTF2 Monoclonal antibody (66063-1-Ig)

    WB analysis of HeLa using 66063-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NTF2 Monoclonal antibody (66063-1-Ig)

    WB analysis of HepG2 using 66063-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    WB analysis of human heart using 66063-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    IHC staining of human placenta using 66063-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    IHC staining of human placenta using 66063-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    IHC staining of human ovary tumor using 66063-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    IHC staining of human ovary tumor using 66063-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    IHC staining of human breast cancer using 66063-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NTF2 Monoclonal antibody (66063-1-Ig)

    IHC staining of human breast cancer using 66063-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHela cells, HepG2 cells, human heart tissue
Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66063-1-Ig is NTF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NTF2 fusion protein Ag7879
Full Name nuclear transport factor 2
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC002348
Gene Symbol NTF2
Gene ID (NCBI) 10204
RRIDAB_11042770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NTF2 antibody 66063-1-IgDownload protocol
IHC protocol for NTF2 antibody 66063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

NTF2 Upregulation in HNSCC: a Predictive Marker and Potential Therapeutic Target Associated With Immune Infiltration.

Authors - Guangxu Xuan
  • KD Validated
View Article