CoraLite® Plus 488-conjugated CD73 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD73 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

CloneNo.

1G5G8

Cat no : CL488-67789

Synonyms

NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase

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Validation Data Gallery

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  IF Staining of human appendicitis using CL488-67789

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite® Plus 488 NT5E/CD73 antibody (CL488-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human appendicitis using CL488-67789

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite® Plus 488 NT5E/CD73 antibody (CL488-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using CL488-67789

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 NT5E/CD73 antibody (CL488-67789, Clone: 1G5G8 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human appendicitis tissue, human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL488-67789 targets CD73 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD73 fusion protein Ag30616
• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: CD73
• Gene ID (NCBI): 4907
• RRID: AB_2919547
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Publications

Species	Application	Title
human	IF	Front Immunol; NT5E upregulation in head and neck squamous cell carcinoma: A novel biomarker on cancer-associated fibroblasts for predicting immunosuppressive tumor microenvironment; Authors - Xue-min Chen
	IF	Front Pharmacol; Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment; Authors - Xin-Miao Xue