CoraLite®594-conjugated NSE Monoclonal antibody

NSE Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6F8G3

Cat no : CL594-66150

Synonyms

ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-66150

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66150 (NSE antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL594-66150

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66150 (NSE antibody) at dilution of 1:50.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66150 is NSE Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSE fusion protein Ag19106
• Full Name: enolase 2 (gamma, neuronal)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC002745
• Gene Symbol: NSE
• Gene ID (NCBI): 2026
• RRID: AB_2883524
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. And this antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.