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CoraLite® Plus 488-conjugated NSD3 Recombinant antibody

NSD3 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230499C5

Cat no : CL488-83116

Synonyms

WHSC1L1, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3, EC:2.1.1.370

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated NSD3 Recombinant ant (CL488-83116)

    IF Staining of MCF-7 using CL488-83116

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 NSD3 antibody (CL488-83116, Clone: 230499C5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated NSD3 Recombinant ant (CL488-83116)

    FC experiment of HeLa using CL488-83116

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Nsd3 Recombinant Antibody (CL488-83116, Clone:230499C5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83116 targets NSD3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSD3 fusion protein Ag1894
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Calculated Molecular Weight 1437 aa, 162 kDa
GenBank Accession NumberBC012059
Gene Symbol WHSC1L1
Gene ID (NCBI) 54904
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NSD3 antibody CL488-83116Download protocol
Standard Protocols
Click here to view our Standard Protocols