NRG1, isoform SMDF Monoclonal antibody, PBS Only

NRG1, isoform SMDF Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat, rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C6D12

Cat no : 66492-1-PBS

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF

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Validation Data Gallery

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  WB analysis using 66492-1-Ig (same clone as 66492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66492-1-Ig (same clone as 66492-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66492-1-Ig (same clone as 66492-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (66492-1-Ig, Clone: 2C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66492-1-PBS targets NRG1, isoform SMDF in WB, IF, Indirect ELISA applications and shows reactivity with Human, pig, mouse, rat, rabbit samples.

• Tested Reactivity: Human, pig, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NRG1, isoform SMDF fusion protein Ag0803
• Full Name: neuregulin 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007675
• Gene Symbol: NRG1
• Gene ID (NCBI): 3084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.