CoraLite® Plus 488-conjugated NRF1/nuclear respiratory factor 1 Recombinant antibody

NRF1/nuclear respiratory factor 1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230394B1

Cat no : CL488-83092

Synonyms

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-83092

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NRF1/nuclear respiratory factor 1 antibody (CL488-83092, Clone: 230394B1 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83092 targets NRF1/nuclear respiratory factor 1 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NRF1/nuclear respiratory factor 1 fusion protein Ag3172
• Full Name: nuclear respiratory factor 1
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC016925
• Gene Symbol: NRF1
• Gene ID (NCBI): 4899
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.