NRAS Polyclonal antibody

• Featured Product
• KD/KO Validated

NRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

33

Reviews (1)

Cat no : 10724-1-AP

Synonyms

HRAS1, Transforming protein N Ras, NRAS1, N ras, GTPase NRas

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Validation Data Gallery

• 

  WB analysis using 10724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10724-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10724-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10724-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10724-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NRAS antibody (10724-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 10724-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NRAS (10724-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, C6 cells, NIH/3T3 cells, Jurkat cells, A549 cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human pancreas tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

10724-1-AP targets NRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRAS fusion protein Ag1081
• Full Name: neuroblastoma RAS viral (v-ras) oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC005219
• Gene Symbol: NRAS
• Gene ID (NCBI): 4893
• RRID: AB_2154209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).

Publications

Species	Application	Title
human	IF	Nat Cell Biol; ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels.; Authors - Xue Hao
human	WB	Gut; RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes.; Authors - Jiazeng Sun
human	WB	Nat Commun; Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma; Authors - Perla Pucci
human	IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human，mouse	WB	Biomaterials; Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.; Authors - Yuanyuan Liu
human	WB	Clin Cancer Res; Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.; Authors - Chen Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brandon (Verified Customer) (11-27-2018)

Worked excellent on mouse tissue from inducible tumor model!

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Tumor Tissue

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