NR1H3 Monoclonal antibody, PBS Only

NR1H3 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A4F7

Cat no : 60134-1-PBS

Synonyms

Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1

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Validation Data Gallery

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  WB analysis using 60134-1-Ig (same clone as 60134-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60134-1-Ig (same clone as 60134-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60134-1-Ig (same clone as 60134-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60134-1-Ig (same clone as 60134-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60134-1-Ig (NR1H3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60134-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60134-1-PBS targets NR1H3 in WB, IF, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NR1H3 fusion protein Ag6184
• Full Name: nuclear receptor subfamily 1, group H, member 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC041172
• Gene Symbol: NR1H3
• Gene ID (NCBI): 10062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.