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NR1H3 Monoclonal antibody

NR1H3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A4F7

Cat no : 60134-1-Ig

Synonyms

Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NR1H3 Monoclonal antibody (60134-1-Ig)

    WB analysis using 60134-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NR1H3 Monoclonal antibody (60134-1-Ig)

    WB analysis of MCF-7 using 60134-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NR1H3 Monoclonal antibody (60134-1-Ig)

    WB analysis of HepG2 using 60134-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NR1H3 Monoclonal antibody (60134-1-Ig)

    IF Staining of HepG2 using 60134-1-Ig

    Immunofluorescent analysis of HepG2 cells using 60134-1-Ig (NR1H3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

Tested Applications

Positive WB detected inT-47D cells, HepG2 cells, MCF-7 cells, COLO 320 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60134-1-Ig targets NR1H3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR1H3 fusion protein Ag6184
Full Name nuclear receptor subfamily 1, group H, member 3
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC041172
Gene Symbol NR1H3
Gene ID (NCBI) 10062
RRIDAB_2154887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.

Protocols

Product Specific Protocols
WB protocol for NR1H3 antibody 60134-1-IgDownload protocol
IF protocol for NR1H3 antibody 60134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Food Funct

CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.

Authors - Xin Shen
View Article
humanIHC

J Transl Med

Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer

Authors - Wei Tang
View Article