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NQO2 Monoclonal antibody, PBS Only

NQO2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9B5

Cat no : 68487-1-PBS

Synonyms

DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of rabbit kidney tissue using NQO2 Monoclonal antibody (68487-1-Ig)

    WB analysis of rabbit kidney using 68487-1-Ig (same clone as 68487-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68487-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NQO2 Monoclonal antibody (68487-1-Ig)

    WB analysis using 68487-1-Ig (same clone as 68487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68487-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68487-1-PBS targets NQO2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Reactivity Human, mouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NQO2 fusion protein Ag8156
Full Name NAD(P)H dehydrogenase, quinone 2
Calculated Molecular Weight 231 aa, 26 kDa
GenBank Accession NumberBC006096
Gene Symbol NQO2
Gene ID (NCBI) 4835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.