NQO2 Monoclonal antibody

NQO2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D9B5

Cat no : 68487-1-Ig

Synonyms

DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2

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Validation Data Gallery

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  WB analysis of rabbit kidney using 68487-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68487-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, rabbit kidney tissue, HeLa, K-562, TF-1

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68487-1-Ig targets NQO2 in WB, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

• Tested Reactivity: Human, mouse, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NQO2 fusion protein Ag8156
• Full Name: NAD(P)H dehydrogenase, quinone 2
• Calculated Molecular Weight: 231 aa, 26 kDa
• GenBank Accession Number: BC006096
• Gene Symbol: NQO2
• Gene ID (NCBI): 4835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.