NQO1 Monoclonal antibody, PBS Only

NQO1 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1E5G7

Cat no : 67240-1-PBS

Synonyms

DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase

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Validation Data Gallery

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  WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67240-1-Ig (same clone as 67240-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67240-1-Ig (same clone as 67240-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NQO1 (67240-1-Ig, Clone:1E5G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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Product Information

67240-1-PBS targets NQO1 in Indirect ELISA, IHC, WB applications and shows reactivity with rat, human samples.

• Tested Reactivity: rat, human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NQO1 fusion protein Ag28933
• Full Name: NAD(P)H dehydrogenase, quinone 1
• Calculated Molecular Weight: 274 aa, 31 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC007659
• Gene Symbol: NQO1
• Gene ID (NCBI): 1728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )