NOX2 Recombinant antibody, PBS Only

NOX2 Uni-rAb^TM Recombinant Antibody for WB, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B17

Cat no : 82872-1-PBS

Synonyms

CYBB, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox, CGD

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Validation Data Gallery

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  WB analysis using 82872-1-RR (same clone as 82872-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82872-1-RR (CYBB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82872-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 82872-1-RR (same clone as 82872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82872-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 82872-1-RR (same clone as 82872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82872-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 82872-1-RR (same clone as 82872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82872-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 82872-1-RR (same clone as 82872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82872-1-PBS in a different storage buffer formulation.

• 

  FC experiment of RAW 264.7 using 82872-1-RR (same clone as 82872-1-PBS)

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug NOX2 Recombinant antibody (82872-1-RR, Clone:1B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82872-1-PBS in a different storage buffer formulation.

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Product Information

82872-1-PBS targets NOX2 in WB, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOX2 fusion protein Ag4402
• Full Name: cytochrome b-245, beta polypeptide
• Calculated Molecular Weight: 577 aa, 65 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032720
• Gene Symbol: NOX2
• Gene ID (NCBI): 1536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).