NONO Monoclonal antibody, PBS Only

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  WB analysis of C2C12 using 66361-1-Ig (same clone as 66361-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66361-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66361-1-Ig (same clone as 66361-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66361-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 66361-1-Ig (same clone as 66361-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66361-1-PBS in a different storage buffer formulation.

NONO Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66361-1-PBS

Clone No.2A2B10

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, Indirect ELISA

Synonyms

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB

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Product Information

66361-1-PBS targets NONO in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NONO fusion protein Ag17609
• Full Name: non-POU domain containing, octamer-binding
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010049
• Gene Symbol: NONO
• Gene ID (NCBI): 4841
• RRID: AB_2881741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15233
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.