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NONO Monoclonal antibody

NONO Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A2B10

Cat no : 66361-1-Ig

Synonyms

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB

Validation Data Gallery

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  • Western Blot (WB) analysis of C2C12 cells using NONO Monoclonal antibody (66361-1-Ig)

    WB analysis of C2C12 using 66361-1-Ig

    C2C12 cell were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using NONO Monoclonal antibody (66361-1-Ig)

    WB analysis of ROS1728 using 66361-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NONO Monoclonal antibody (66361-1-Ig)

    WB analysis of mouse skeletal muscle using 66361-1-Ig

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inC2C12 cell, mouse skeletal muscle tissue, ROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66361-1-Ig targets NONO in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NONO fusion protein Ag17609
Full Name non-POU domain containing, octamer-binding
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC010049
Gene Symbol NONO
Gene ID (NCBI) 4841
RRIDAB_2881741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Protocols

Product Specific Protocols
WB protocol for NONO antibody 66361-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Circular RNA circTmem241 drives group III innate lymphoid cell differentiation via initiation of Elk3 transcription

Authors - Nian Liu
View Article