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CoraLite® Plus 488-conjugated NOL11 Recombinant antibody

NOL11 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240371B5

Cat no : CL488-83391-6

Synonyms

Nucleolar protein 11, L14, 240371B5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated NOL11 Recombinant an (CL488-83391-6)

    IF Staining of HeLa using CL488-83391-6

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NOL11 antibody (CL488-83391-6, Clone: 240371B5 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83391-6 is NOL11 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NOL11 fusion protein Ag11783
Full Name nucleolar protein 11
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight81 kDa
GenBank Accession NumberBC064404
Gene Symbol NOL11
Gene ID (NCBI) 25926
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NOL11 antibody CL488-83391-6Download protocol
Standard Protocols
Click here to view our Standard Protocols