NOL11 Recombinant antibody, PBS Only

NOL11 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240371B5

Cat no : 83391-6-PBS

Synonyms

Nucleolar protein 11, L14, 240371B5

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Validation Data Gallery

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  WB analysis using 83391-6-RR (same clone as 83391-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83391-6-RR (NOL11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83391-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83391-6-RR (same clone as 83391-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83391-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83391-6-RR (same clone as 83391-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83391-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83391-6-RR (same clone as 83391-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOL11 Recombinant antibody (83391-6-RR, Clone:240371B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83391-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83391-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83391-6-RR against Human NOL11 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83391-6-PBS is NOL11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOL11 fusion protein Ag11783
• Full Name: nucleolar protein 11
• Calculated Molecular Weight: 719 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC064404
• Gene Symbol: NOL11
• Gene ID (NCBI): 25926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.