NODAL Polyclonal antibody, PBS Only

NODAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 27430-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 27430-1-AP (NODAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293T using 27430-1-AP (same clone as 27430-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human NODAL (27430-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

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Product Information

27430-1-PBS targets NODAL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NODAL fusion protein Ag26729
• Full Name: nodal homolog (mouse)
• Calculated Molecular Weight: 347 aa, 40 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC104976
• Gene Symbol: NODAL
• Gene ID (NCBI): 4838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nodal is a member of the transforming growth factor-β (TGF-β) superfamily that plays critical roles during embryogenesis. Nodal also regulates cell proliferation, apoptosis, and invasion in cancer cells. However, it appears to exert both tumor-suppressing and tumor-promoting effects, depending on the cell type (PMID: 22645523). Nodal signals through ALK7 receptor to inhibit proliferation and to induce apoptosis in ovarian cancer cell lines and breast cancer cell lines (PMID: 15531507, PMID: 21383881).